Cell and Tissue Culture Dishes are ideal containers for cell growth and yields aim on little and medium input volume, and also useful in sample separation, pre-treatment, storage and so on.
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A tissue culture is an amalgamation of cells or tissues growing separately from the original plant or animal. Scientists use them in order to study the biology of cells or tissues in a closely-monitored, easily-manipulatable environment. A nutrient-rich growth medium provides the cells in these cultures with the sustenance to grow outside of their organism of origin, while the tissue dish is able to contain the mixture of growth medium and tissue culture to protect it from outside factors. In other words, these dishes are fundamental to scientific studies because they keep the tissue culture in a contained environment, away from all outside factors that can act as contaminants for the study.
It is because of tissue culture technology that science has made so many advancements in understanding cellular biology, particularly in its applications to the field of medicine.
The two-dimensional dishes have been used and perfected since 1885, when Wilhelm Roux first incubated a chicken embryo on a plate in a saline solution for several days. With the advancement of polymer technology, which allowed scientists to manufacture plastics, Julius Richard Petri was able to create a more perfect vessel that is still widely used today: the Petri dish. Two-dimensional vessels have been used in a variety of fields and studies, notably stem-cell research, cell lineage specification studies, cancer research, and hepatocyte function studies. Even despite the development of three-dimensional dishes and tissue culture systems, these flat surfaces have remained incredibly important in many fields, and they have yet to be replaced entirely by their three-dimensional counterparts.
Nonetheless, three-dimensional vessels for tissue cultures have proven to be extremely interesting for studying the intercellular interaction in an environment, and moreover, providing a more realistic model of cell development. In a three-dimensional culture system, cells are able to grow in any direction, providing researchers with a a better understanding of their natural growth patterns and cell-cell matrix development as they grow in living organisms. The importance of three-dimensional tissue cultures was first demonstrated by studies in the 1980s under the direction of Mina Bissell, and this work showed that given the opportunity, cells could form multicellular structures in a culture. Further studies have showed that the genetic expressions of cells is different when grown in two-dimensional versus three-dimensional environments.
In addition to the shape of the vessel, researchers are able to find vessels that match the conditions of their experiments. For instance, they can buy tissue culture vessels made from different polymers, which can in turn control factors like the amount of oxygen that reaches the culture, or help prevent the degradation of vessels in the presence of reactive solvents that are used to treat the culture for analysis. There are also pre-treated vessels, which depending on the trademarked treatment, provide different methods to promote better cell adhesion.
Useful Resources to Learn More about Tissue Cultures
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